paper n a db db mice (Jackson Laboratory)
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paper n a db db mice
Paper N A Db Db Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paper+n+a+mouse/db+db+mice/pm41950006-243-53-58
Average 86 stars, based on 1 article reviews
Paper N A Db Db Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paper+n+a+mouse/db+db+mice/pm41950006-243-53-58
Average 86 stars, based on 1 article reviews
paper n a db db mice - by Bioz Stars,
2026-11
86/100 stars
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Quantitative RT-PCR:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This CRISPR:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. 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REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This Negative Control:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This BAC Assay:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This Clone Assay:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This Software:Article Title: Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cells. Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081058 Alt-R Cas9 Electroporation Enhancer IDT Cat# 1075915 (Z)-4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 Recombinant Mouse GM-CSF (carrier free) BioLegend Cat# 576304 Recombinant Mouse IL-4 (carrier free) BioLegend Cat# 574304 Collagenase D from Clostridium histolyticum Roche Cat# 11088858001 Pronase from Streptomyces griseus Millipore Sigma Cat# 10165921001 Amphotericin B solution Millipore Sigma Cat# A2942 Liberase TL Roche Cat# 5401020001 Bovine Serum Albumin, Powder Millipore Sigma Cat# A9647 Deoxyribonuclease I (DNase I) from bovine pancreas Roche Cat# 11284932001 RBC Lysis Buffer BioLegend Cat# 420301 Poly(I:C) high molecular weight InvivoGen Cat# tlrl-pic CpG-A/ODN 2216 InvivoGen Cat# tlrl-2216-1 DOTAP chloride MedChemExpress Cat# HY-112754A Concanavalin A from Canavalia ensiformis Millipore Sigma Cat# L7647, C2010 Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences Cat# 15710 Triton X-100 Thermo Fisher Scientific Cat# 85111 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease Inhibitor Cocktail Thermo Fisher Scientific Cat# 78429 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78420 NuPAGE LDS Sample Buffer Thermo Fisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent Thermo Fisher Scientific Cat# NP0009 TRIzol Reagent Themo Fisher Scientific Cat# 15596018 Digitonin Thermo Fisher Scientific Cat# BN2006 Trizma Hydrochloride Solution, pH 7.4 Millipore Sigma Cat# T2194 Sodium Chloride Solution, 5 M Millipore Sigma Cat# 59222C Magnesium Chloride Solution, 1M Millipore Sigma Cat# M1028 IGEPAL CA-630 Millipore Sigma Cat# I8896 Protector RNase inhibitor Millipore Sigma Cat# 3335402001 DL-Dithiothreitol (DTT) solution, 1M Millipore Sigma Cat# 646563 Bovine Serum Albumin Fraction V Solution Thermo Fisher Scientific Cat# 15260037 10% Tween 20 Bio-Rad Cat# 1662404 Critical commercial assays Venor GeM Mycoplasma PCR-Based Detection Kit Sigma-Aldrich Cat# MP0025 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 KT Streptavidin microbeads Miltenyi Biotec Cat# 130-048-101 LD columns Miltenyi Biotec Cat# 130-042-901 (Continued on next page) Immunity 59, 270–287.e1–e11, February 10, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Combined snRNA-Seq and snATAC-Seq primary splenic pDCs Pucella et al. 72 GEO: GSE252189 Experimental models: Cell lines Mouse: L929 ATCC Cat# CCL-1, RRID:CVCL_0462 Mouse: B16-Flt3L Mach et al. 82 N/A Mouse: HoxB8-FL Redecke et al. 83 N/A Mouse: Pmi28 c/o C. Cardoso N/A African green monkey: Vero E6 ATCC Cat# CRL1586; RRID: CVCL_0574 Experimental models: Organisms/strains Mouse: Smc3 fl : B6(SJL)Smc3 tm1c(EUCOMM)Wtsi /J The Jackson Laboratory RRID: IMSR_JAX:030559 Mouse: Rosa26 CreERT2 Dr. Thomas Ludwig, Ohio State University N/A Mouse: Ifn ΔTB2 This Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Rhod-2 AM Cayman Chemical CAS: 145037-81-6 (−)-Isoproterenol hydrochloride Sigma CAS: 5984-95-2 Ru360 Sigma CAS: 557440 Experimental models: Cell lines Human: HEK293T ATTC https://www.atcc.org/products/all/crl-3216.aspx#generalinformation Experimental models: Organisms/strains Mouse: MCU-KO line Pan, et al 2013 N/A Mouse: TOMM20-mNeonGreen This Article Title: PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER viFSP1 Vistas-lab Cat# STK626779 icFSP1 Intonation Research Laboratories N/A Imidazole ketone erastin (IKE) Selleckchem Cat# S8877 Liproxstatin-1 (Lip-1) Selleckchem Cat# S7699 L-Glutathione (GSH) Sigma Aldrich Cat# G4251 L-Glutathione oxidized (GSSG) WAKO Cat# 79-03333 Seleno-diglutathione (GS-Se-SG) This study N/A N-ethylmaleimide (NEM) Nacalai Tesque Cat# 15512-11 Resazurin sodium salt Sigma Aldrich Cat# R7017 Sodium selenite (Na2SeO3) Sigma Aldrich Cat# S5261 L-Selenocystine (Sec2) Sigma Aldrich Cat# 545996 L-Selenomethionine (SeM) Sigma Aldrich Cat# S3132 MJ33 Cayman Cat# 90001844 Rose bengal Wako Cat# 184-00272 PEI MAX Polysciences Cat# 24765 1-stearoyl-2-linoleoyl-sn-glycero-3phosphoethanolamine (16:0/18:2 PE) Avanti Cat# 850802C Iodomethane-d3 Isotec Cat# 176036 Deuterium-labelled phosphatidylcholine hydroperoxide (PCOOH-d9) This study N/A Insulin Gibco Cat# 12585014 Transferrin Sigma Aldrich Cat# T3309 Recombinant human PRDX6 This study N/A Recombinant human PRDX6 C47S This study N/A Critical commercial assays Cytotoxicity Detection kit (LDH assay kit) Roche Cat# 11644793001 KOD -plus- Mutagenesis Kit TOYOBO Cat# SMK-101 Deposited data Western blots and microscopy images This study Mendeley Data: https://doi.org/ 10.17632/d2wzfr4twp.3 Experimental models: Cell lines Human: HEK293T ATCC CRL-1619 Human: HT-1080 ATCC CCL-121 Human: 786-O ATCC CRL-1932 Human: A375 ATCC CRL-1619 Human: A549 ATCC CCL-185 Human: NCI-H460 ATCC HTB-177 Human: LOX-IMVI NCI/NIH CVCL_1381 Human: HEC151 JCRB JCRB1122-A Human: HT-1080 GPX4 KO Mishima et al.14 N/A Human: HT-1080 GPX4 KO + hGPX4 short form OE Mishima et al.67 N/A Human: HT-1080 PRDX6 KO This paper N/A Human: HT-1080 PRDX1 KO This paper N/A Human: HT-1080 PRDX2 KO This paper N/A Human: HT-1080 PRDX3 KO This paper N/A Human: HT-1080 PRDX4 KO This paper N/A Human: HT-1080 PRDX5 KO This paper N/A Human: HT-1080 PRDX6 KO + hPRDX6 OE This paper N/A Human: HT-1080 PRDX6 KO + HA-hPRDX6 OE This paper N/A (Continued on next page) e2 Molecular Cell 84, 1–16.e1–e9, December 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: 786-O PRDX6 KO This paper N/A Human: A375 PRDX6 KO This paper N/A Human: A549 PRDX6 KO This paper N/A Human: NCI-H460 PRDX6 KO This paper N/A Human: LOX-IMVI PRDX6 KO This paper N/A Human: HEC151 PRDX6 KO This paper N/A 4-hydroxytamoxifen inducible Gpx4 knockout MEFs (Pfa1 cells) Seiler et al.31 N/A Mouse: Pfa1 Gpx4 KO Nakamura et al.30 N/A Mouse: Pfa1 Gpx4 KO + HA-mPRDX6 OE This Article Title: Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Norepinephrine ELISA kit Abcam Catalog# AB287789 EasySepTM Mouse CD8+ T Cell Isolation Kit STEMCELL Technologies Catalog# 19853 Chromium Single Cell 3′ Library & Gel Bead Kit v1 10X Genomics Catalog# PN-120237 Experimental Models: Organisms/Strains Mouse: C57BL6/J The Jackson Laboratory JAX ID #:000664 Mouse: TH-CreER Abraira et al.123 MGI ID #: 5569743 Mouse: Adrb2f/f Hinoi et al.124 MGI ID #:3837346 Mouse: K15-rtTA This other:Article Title: PHD3 Loss Promotes Exercise Capacity and Fat Oxidation in Skeletal Muscle. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER L-Carnitine Sigma-Aldrich Cat# C0158 Palmitic acid Sigma-Aldrich Cat# P0500 1 mCi [9,10-3H(N)]-palmitic acid Perkin Elmer Cat# NET043001MC Palmitate-BSA FAO Substrate Agilent Technologies Cat# 102720-100 Hydrogen peroxide solution 30% Sigma-Aldrich Cat# H1009 Western Lightning ECL Pro Perkin Elmer Cat# NEL120001EA ECL Plus Western Blotting Substrate Thermo Scientific Cat# 32132 Commercial Assays Liquid DAB+ Substrate Chromogen System DAKO Cat# K346811-2 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-11268 Immortalized MEF ATCC Cat# CRL-2907 C2C12 ATCC Cat# CRL-1772 AMPKa KO and WT MEFs From the laboratory of Dr. Reuben Shaw N/A Experimental Models: Organisms/Strains Mouse: AMPKFL;Ubc-CreER From the laboratory of Dr. Reuben Shaw N/A Mouse: PHD3FL:MCK-Cre, PHD3FL:CMV-Cre and PHD3FL This paper N/A Mouse: Article Title: Scap stabilizes PKM2 to promote glycolysis and enhance anti-fungal immunity in macrophages. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TOFA MedChemExpress Cat#HY-101068 Betulin MedChemExpress Cat#HY-N0083 Mg132 MedChemExpress Cat#HY-13259 Chloroquine(CQ) MedChemExpress Cat#HY-17589A CyCloheximide MedChemExpress Cat#HY-12320 2-DG MedChemExpress Cat#HY-13966 DASA58 Selleck Cat#S7928 D-Glucose(U-13C6,99%) Cambridge Isotope Laboratories Cat#CLM-1396-0.5 BODIPYTM 493/503 Thermo Fisher Scientific Cat#D3922 Critical commercial assays Glycolysis Stress Test Kit Agilent Technologies Cat#103020-100 Cell Mito Stress Test Kit Agilent Technologies Cat#103015-100 Mouse IL-1β ELISA Kit Youkelife Cat#EM0001 Mouse IL-6 ELISA Kit Youkelife Cat#EM0004 Mouse TNFα ELISA Kit Youkelife Cat#EM0010 Deposited data RNA-seq data of mouse bone marrow derived macrophages This paper GEO: GSE288513 RNA-seq data of human leukocytes Le KTT et al.24 GEO: GSE131590 RNA-seq data of human monocytes Häder A et al.25 GEO: GSE177040 RNA-seq data of mouse bone marrow derived macrophages De Castro RJA et al.26 GEO: GSE243519 Experimental models: Cell lines Human: HEK293T ATCC Cat#CRL-11268 Experimental models: Organisms/strains Mouse: Article Title: Gut microbe-derived metabolites drive psoriatic inflammation via modulation of skin Th17 cells. Article Snippet: Article Gut microbe-derived metabolites drive psoriatic inflammation via modulation of skin Th17 cells Control:Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Recombinant:Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Rhod-2 AM Cayman Chemical CAS: 145037-81-6 (−)-Isoproterenol hydrochloride Sigma CAS: 5984-95-2 Ru360 Sigma CAS: 557440 Experimental models: Cell lines Human: HEK293T ATTC https://www.atcc.org/products/all/crl-3216.aspx#generalinformation Experimental models: Organisms/strains Mouse: MCU-KO line Pan, et al 2013 N/A Mouse: TOMM20-mNeonGreen This Plasmid Preparation:Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Rhod-2 AM Cayman Chemical CAS: 145037-81-6 (−)-Isoproterenol hydrochloride Sigma CAS: 5984-95-2 Ru360 Sigma CAS: 557440 Experimental models: Cell lines Human: HEK293T ATTC https://www.atcc.org/products/all/crl-3216.aspx#generalinformation Experimental models: Organisms/strains Mouse: MCU-KO line Pan, et al 2013 N/A Mouse: TOMM20-mNeonGreen This Microscopy:Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article DNA Extraction:Article Title: Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Rhod-2 AM Cayman Chemical CAS: 145037-81-6 (−)-Isoproterenol hydrochloride Sigma CAS: 5984-95-2 Ru360 Sigma CAS: 557440 Experimental models: Cell lines Human: HEK293T ATTC https://www.atcc.org/products/all/crl-3216.aspx#generalinformation Experimental models: Organisms/strains Mouse: MCU-KO line Pan, et al 2013 N/A Mouse: TOMM20-mNeonGreen This Spectroscopy:Article Title: Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Rhod-2 AM Cayman Chemical CAS: 145037-81-6 (−)-Isoproterenol hydrochloride Sigma CAS: 5984-95-2 Ru360 Sigma CAS: 557440 Experimental models: Cell lines Human: HEK293T ATTC https://www.atcc.org/products/all/crl-3216.aspx#generalinformation Experimental models: Organisms/strains Mouse: MCU-KO line Pan, et al 2013 N/A Mouse: TOMM20-mNeonGreen This Imaging:Article Title: Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Rhod-2 AM Cayman Chemical CAS: 145037-81-6 (−)-Isoproterenol hydrochloride Sigma CAS: 5984-95-2 Ru360 Sigma CAS: 557440 Experimental models: Cell lines Human: HEK293T ATTC https://www.atcc.org/products/all/crl-3216.aspx#generalinformation Experimental models: Organisms/strains Mouse: MCU-KO line Pan, et al 2013 N/A Mouse: TOMM20-mNeonGreen This Knock-Out:Article Title: PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER viFSP1 Vistas-lab Cat# STK626779 icFSP1 Intonation Research Laboratories N/A Imidazole ketone erastin (IKE) Selleckchem Cat# S8877 Liproxstatin-1 (Lip-1) Selleckchem Cat# S7699 L-Glutathione (GSH) Sigma Aldrich Cat# G4251 L-Glutathione oxidized (GSSG) WAKO Cat# 79-03333 Seleno-diglutathione (GS-Se-SG) This study N/A N-ethylmaleimide (NEM) Nacalai Tesque Cat# 15512-11 Resazurin sodium salt Sigma Aldrich Cat# R7017 Sodium selenite (Na2SeO3) Sigma Aldrich Cat# S5261 L-Selenocystine (Sec2) Sigma Aldrich Cat# 545996 L-Selenomethionine (SeM) Sigma Aldrich Cat# S3132 MJ33 Cayman Cat# 90001844 Rose bengal Wako Cat# 184-00272 PEI MAX Polysciences Cat# 24765 1-stearoyl-2-linoleoyl-sn-glycero-3phosphoethanolamine (16:0/18:2 PE) Avanti Cat# 850802C Iodomethane-d3 Isotec Cat# 176036 Deuterium-labelled phosphatidylcholine hydroperoxide (PCOOH-d9) This study N/A Insulin Gibco Cat# 12585014 Transferrin Sigma Aldrich Cat# T3309 Recombinant human PRDX6 This study N/A Recombinant human PRDX6 C47S This study N/A Critical commercial assays Cytotoxicity Detection kit (LDH assay kit) Roche Cat# 11644793001 KOD -plus- Mutagenesis Kit TOYOBO Cat# SMK-101 Deposited data Western blots and microscopy images This study Mendeley Data: https://doi.org/ 10.17632/d2wzfr4twp.3 Experimental models: Cell lines Human: HEK293T ATCC CRL-1619 Human: HT-1080 ATCC CCL-121 Human: 786-O ATCC CRL-1932 Human: A375 ATCC CRL-1619 Human: A549 ATCC CCL-185 Human: NCI-H460 ATCC HTB-177 Human: LOX-IMVI NCI/NIH CVCL_1381 Human: HEC151 JCRB JCRB1122-A Human: HT-1080 GPX4 KO Mishima et al.14 N/A Human: HT-1080 GPX4 KO + hGPX4 short form OE Mishima et al.67 N/A Human: HT-1080 PRDX6 KO This paper N/A Human: HT-1080 PRDX1 KO This paper N/A Human: HT-1080 PRDX2 KO This paper N/A Human: HT-1080 PRDX3 KO This paper N/A Human: HT-1080 PRDX4 KO This paper N/A Human: HT-1080 PRDX5 KO This paper N/A Human: HT-1080 PRDX6 KO + hPRDX6 OE This paper N/A Human: HT-1080 PRDX6 KO + HA-hPRDX6 OE This paper N/A (Continued on next page) e2 Molecular Cell 84, 1–16.e1–e9, December 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: 786-O PRDX6 KO This paper N/A Human: A375 PRDX6 KO This paper N/A Human: A549 PRDX6 KO This paper N/A Human: NCI-H460 PRDX6 KO This paper N/A Human: LOX-IMVI PRDX6 KO This paper N/A Human: HEC151 PRDX6 KO This paper N/A 4-hydroxytamoxifen inducible Gpx4 knockout MEFs (Pfa1 cells) Seiler et al.31 N/A Mouse: Pfa1 Gpx4 KO Nakamura et al.30 N/A Mouse: Pfa1 Gpx4 KO + HA-mPRDX6 OE This Targeted Proteomics:Article Title: PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER viFSP1 Vistas-lab Cat# STK626779 icFSP1 Intonation Research Laboratories N/A Imidazole ketone erastin (IKE) Selleckchem Cat# S8877 Liproxstatin-1 (Lip-1) Selleckchem Cat# S7699 L-Glutathione (GSH) Sigma Aldrich Cat# G4251 L-Glutathione oxidized (GSSG) WAKO Cat# 79-03333 Seleno-diglutathione (GS-Se-SG) This study N/A N-ethylmaleimide (NEM) Nacalai Tesque Cat# 15512-11 Resazurin sodium salt Sigma Aldrich Cat# R7017 Sodium selenite (Na2SeO3) Sigma Aldrich Cat# S5261 L-Selenocystine (Sec2) Sigma Aldrich Cat# 545996 L-Selenomethionine (SeM) Sigma Aldrich Cat# S3132 MJ33 Cayman Cat# 90001844 Rose bengal Wako Cat# 184-00272 PEI MAX Polysciences Cat# 24765 1-stearoyl-2-linoleoyl-sn-glycero-3phosphoethanolamine (16:0/18:2 PE) Avanti Cat# 850802C Iodomethane-d3 Isotec Cat# 176036 Deuterium-labelled phosphatidylcholine hydroperoxide (PCOOH-d9) This study N/A Insulin Gibco Cat# 12585014 Transferrin Sigma Aldrich Cat# T3309 Recombinant human PRDX6 This study N/A Recombinant human PRDX6 C47S This study N/A Critical commercial assays Cytotoxicity Detection kit (LDH assay kit) Roche Cat# 11644793001 KOD -plus- Mutagenesis Kit TOYOBO Cat# SMK-101 Deposited data Western blots and microscopy images This study Mendeley Data: https://doi.org/ 10.17632/d2wzfr4twp.3 Experimental models: Cell lines Human: HEK293T ATCC CRL-1619 Human: HT-1080 ATCC CCL-121 Human: 786-O ATCC CRL-1932 Human: A375 ATCC CRL-1619 Human: A549 ATCC CCL-185 Human: NCI-H460 ATCC HTB-177 Human: LOX-IMVI NCI/NIH CVCL_1381 Human: HEC151 JCRB JCRB1122-A Human: HT-1080 GPX4 KO Mishima et al.14 N/A Human: HT-1080 GPX4 KO + hGPX4 short form OE Mishima et al.67 N/A Human: HT-1080 PRDX6 KO This paper N/A Human: HT-1080 PRDX1 KO This paper N/A Human: HT-1080 PRDX2 KO This paper N/A Human: HT-1080 PRDX3 KO This paper N/A Human: HT-1080 PRDX4 KO This paper N/A Human: HT-1080 PRDX5 KO This paper N/A Human: HT-1080 PRDX6 KO + hPRDX6 OE This paper N/A Human: HT-1080 PRDX6 KO + HA-hPRDX6 OE This paper N/A (Continued on next page) e2 Molecular Cell 84, 1–16.e1–e9, December 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: 786-O PRDX6 KO This paper N/A Human: A375 PRDX6 KO This paper N/A Human: A549 PRDX6 KO This paper N/A Human: NCI-H460 PRDX6 KO This paper N/A Human: LOX-IMVI PRDX6 KO This paper N/A Human: HEC151 PRDX6 KO This paper N/A 4-hydroxytamoxifen inducible Gpx4 knockout MEFs (Pfa1 cells) Seiler et al.31 N/A Mouse: Pfa1 Gpx4 KO Nakamura et al.30 N/A Mouse: Pfa1 Gpx4 KO + HA-mPRDX6 OE This Epinephrine ELISA:Article Title: Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Norepinephrine ELISA kit Abcam Catalog# AB287789 EasySepTM Mouse CD8+ T Cell Isolation Kit STEMCELL Technologies Catalog# 19853 Chromium Single Cell 3′ Library & Gel Bead Kit v1 10X Genomics Catalog# PN-120237 Experimental Models: Organisms/Strains Mouse: C57BL6/J The Jackson Laboratory JAX ID #:000664 Mouse: TH-CreER Abraira et al.123 MGI ID #: 5569743 Mouse: Adrb2f/f Hinoi et al.124 MGI ID #:3837346 Mouse: K15-rtTA This Cell Isolation:Article Title: Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Norepinephrine ELISA kit Abcam Catalog# AB287789 EasySepTM Mouse CD8+ T Cell Isolation Kit STEMCELL Technologies Catalog# 19853 Chromium Single Cell 3′ Library & Gel Bead Kit v1 10X Genomics Catalog# PN-120237 Experimental Models: Organisms/Strains Mouse: C57BL6/J The Jackson Laboratory JAX ID #:000664 Mouse: TH-CreER Abraira et al.123 MGI ID #: 5569743 Mouse: Adrb2f/f Hinoi et al.124 MGI ID #:3837346 Mouse: K15-rtTA This Single Cell:Article Title: Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Norepinephrine ELISA kit Abcam Catalog# AB287789 EasySepTM Mouse CD8+ T Cell Isolation Kit STEMCELL Technologies Catalog# 19853 Chromium Single Cell 3′ Library & Gel Bead Kit v1 10X Genomics Catalog# PN-120237 Experimental Models: Organisms/Strains Mouse: C57BL6/J The Jackson Laboratory JAX ID #:000664 Mouse: TH-CreER Abraira et al.123 MGI ID #: 5569743 Mouse: Adrb2f/f Hinoi et al.124 MGI ID #:3837346 Mouse: K15-rtTA This Knock-In:Article Title: Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Norepinephrine ELISA kit Abcam Catalog# AB287789 EasySepTM Mouse CD8+ T Cell Isolation Kit STEMCELL Technologies Catalog# 19853 Chromium Single Cell 3′ Library & Gel Bead Kit v1 10X Genomics Catalog# PN-120237 Experimental Models: Organisms/Strains Mouse: C57BL6/J The Jackson Laboratory JAX ID #:000664 Mouse: TH-CreER Abraira et al.123 MGI ID #: 5569743 Mouse: Adrb2f/f Hinoi et al.124 MGI ID #:3837346 Mouse: K15-rtTA This Polymerase Chain Reaction:Article Title: Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunity. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Norepinephrine ELISA kit Abcam Catalog# AB287789 EasySepTM Mouse CD8+ T Cell Isolation Kit STEMCELL Technologies Catalog# 19853 Chromium Single Cell 3′ Library & Gel Bead Kit v1 10X Genomics Catalog# PN-120237 Experimental Models: Organisms/Strains Mouse: C57BL6/J The Jackson Laboratory JAX ID #:000664 Mouse: TH-CreER Abraira et al.123 MGI ID #: 5569743 Mouse: Adrb2f/f Hinoi et al.124 MGI ID #:3837346 Mouse: K15-rtTA This |